SAVE NOW with 10% off all Recombinant Antibodies Shop Now

Catalog No: OKEH02322
Size:96T
Price: $525.00
SKU
OKEH02322
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.

DBT ELISA Kit (Human) : 96 Wells (OKEH02322)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology DBT ELISA Kit (Human) (OKEH02322) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DBT has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DBT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DBT captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 91%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.9% (n = 20)
Mean Inter-assay CV%: < 8.1% (n = 20)
ELISA Kit Component
ComponentAmount
DBT Microplate96 Wells (12 x 8 Well strips)
DBT Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated HCN4 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.13 ng/mL
SpecificityNatural and recombinant Human Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolDBT
Gene Full Namedihydrolipoamide branched chain transacylase E2
Alias Symbols52 kDa mitochondrial autoantigen of primary biliary cirrhosis;BCATE2;BCKAD E2 subunit;BCKADE2;BCKAD-E2;BCKDH-E2;BCOADC-E2;branched chain 2-oxo-acid dehydrogenase complex component E2;branched chain acyltransferase, E2 component;branched-chain alpha-keto acid dehydrogenase complex component E2;dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex;Dihydrolipoamide branched chain transacylase;dihydrolipoyl transacylase;dihydrolipoyllysine-residue (2-methylpropanoyl)transferase;E2;E2 component of branched chain alpha-keto acid dehydrogenase complex;E2B;lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial;lipoamide acyltransferase component of mitochondrial branched-chain alpha-keto acid dehydrogenase complex;mitochondrial branched chain alpha-keto acid dehydrogenase transacylase subunit (E2b).
NCBI Gene Id1629
Protein NameP11182
Description of TargetLipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial
Uniprot IDhttps://www.uniprot.org/uniprot/P11182
Protein Accession #NP_001909.3
Nucleotide Accession #NM_001918.3
Protein Size (# AA)482
Molecular Weight53 kDa
Write Your Own Review
You're reviewing:DBT ELISA Kit (Human) : 96 Wells (OKEH02322)
Your Rating
We found other products you might like!