DAZ2 Antibody (OAGA00816)

Catalog No: OAGA00816

Size:100 ul

Sample (30 ug of whole cell lysate)
A: Molt-4
7.5% SDS PAGE
OAGA00816 diluted at 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DAZ2(OAGA00816) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, WB
• Additional Information: Background: This gene is a member of the DAZ gene family and is a candidate for the human Y-chromosomal azoospermia factor (AZF). Its expression is restricted to premeiotic germ cells, particularly in spermatogonia. It encodes an RNA-binding protein that is important for spermatogenesis. Four copies of this gene are found on chromosome Y within palindromic duplications; one pair of genes is part of the P2 palindrome and the second pair is part of the P1 palindrome. Each gene contains a 2.4 kb repeat including a 72-bp exon, called the DAZ repeat; the number of DAZ repeats is variable and there are several variations in the sequence of the DAZ repeat. Each copy of the gene also contains a 10.8 kb region that may be amplified; this region includes five exons that encode an RNA recognition motif (RRM) domain. This gene contains one copy of the 10.8 kb repeat. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]
  Positive Controls: 293T, A431, HeLa, Molt-4, Raji
  Cellular Localization: Cytoplasm, Nucleus
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 237 of DAZ2 (Uniprot ID#Q13117)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: DAZ2
• Gene Full Name: Deleted in azoospermia protein 2
• Alias Symbols: pDP1678
• NCBI Gene Id: 57055
• Protein Name: Deleted in azoospermia protein 2
• Uniprot ID: Q13117
• Protein Accession #: NP_001005785.1
• Molecular Weight: 63 kDa