CYR61 ELISA Kit (Mouse) (OKCD02486)

Catalog No: OKCD02486

Size:96WELLS

Mouse CYR61 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	92-104%	94-101%	89-102%	92-104%
  EDTA Plasma (n=5)	79-89%	93-105%	82-91%	80-105%
  Heparin Plasma (n=5)	88-102%	94-101%	88-102%	89-102%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 6.25-400pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	92-101	95
  EDTA Plasma (n=5)	81-104	88
  Heparin Plasma(n=5)	81-90	86

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CYR61 Microplate: 96 Wells (12 x 8 Well strips)
  • CYR61 Lyophilized Standard: 2
  • 100X Biotinylated CYR61 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 2.36 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61). No significant cross-reactivity or interference between Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Ccn1
• Gene Full Name: cellular communication network factor 1
• Alias Symbols: 3CH61;AI325051;CCN;CCN family member 1;Cyr6;Cyr61;cysteine rich protein 61;cysteine-rich angiogenic inducer 61;IBP-10;IGF-binding protein 10;Igfbp10;IGFBP-10;insulin-like growth factor-binding protein 10;intermediate early;protein CYR61.
• NCBI Gene Id: 16007
• Protein Name: Protein CYR61
• Description of Target: Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5 (By similarity). CYR61-mediated gene regulation is dependent on heparin-binding (By similarity). Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1 (By similarity). Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-1/beta-5 and cell proliferation through integrin alpha-v/beta-3 (By similarity).
• Uniprot ID: P18406
• Protein Accession #: NP_034646.1
• Nucleotide Accession #: NM_010516.2
• Protein Size (# AA): 379