Catalog No: OKEH03587
Size:96T
Price: $625.00
SKU
OKEH03587
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Rat, Rattus norvegicus | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology CYP27B1 ELISA Kit (Rat) (OKEH03587) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human Cyp27b1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Cyp27b1 and incubated. The Cyp27b1 found in the sample or standards competes with the biotinylated Cyp27b1 for limited binding sites on the immobilized anti-human Cyp27b1 antibody. Excess unbound biotinylated Cyp27b1 and sample or standard Cyp27b1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Cyp27b1 captured in the well and inversely proportional to the amount of Cyp27b1 which was contained in the sample or standard. | ||||||||||||||||||||||
ELISA Kit Range | 0.625-40ng/mL | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
Sensitivity | 0.312 ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Rat 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Competitive ELISA |
Gene Symbol | Cyp27b1 |
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Gene Full Name | cytochrome P450, family 27, subfamily b, polypeptide 1 |
Alias Symbols | 25-hydroxyvitamin D(3) 1-alpha-hydroxylase;25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial;25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial-like;25-OHD-1 alpha-hydroxylase;calcidiol 1-monooxygenase;Cyp40;cytochrome p450 27B1;cytochrome P450 subfamily XXVIIB (25-hydroxyvitamin D-1-alpha-hydroxylase) polypeptide 1;cytochrome P450 subfamily XXVIIB polypeptide 1;cytochrome P450, 40 (25-hydroxyvitamin D3 1 alpha-hydroxylase);cytochrome P450, subfamily 27b, polypeptide 1;cytochrome P450C1 alpha;cytochrome P450VD1-alpha;P450C1 alpha;P450VD1-alpha;VD3 1A hydroxylase. |
NCBI Gene Id | 114700 |
Protein Name | O35132 |
Description of Target | 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial |
Uniprot ID | https://www.uniprot.org/uniprot/O35132 |
Protein Accession # | NP_446215.1 |
Nucleotide Accession # | NM_053763.1 |
Protein Size (# AA) | 501 |
Molecular Weight | 55 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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