Catalog No: OKCD02483
Size:96WELLS
Price: $700.00
SKU
OKCD02483
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for CYP27B1 ELISA Kit (Mouse) (OKCD02483) |
---|
Predicted Species Reactivity | Mouse|Mus musculus | ||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 27B1 (CYP27B1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 27B1 (CYP27B1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 27B1 (CYP27B1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cytochrome P450 27B1 (CYP27B1) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.625-40ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome P450 27B1 (CYP27B1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome P450 27B1 (CYP27B1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
| ||||||||||||||||||||||
Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates, cell lysates and other biological fluids | ||||||||||||||||||||||
Sensitivity | 0.235 ng/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of Cytochrome P450 27B1 (CYP27B1). No significant cross-reactivity or interference between Cytochrome P450 27B1 (CYP27B1) and analogues was observed. | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA | ||||||||||||||||||||||
Protocol Information |
|
Gene Symbol | Cyp27b1 |
---|---|
Gene Full Name | cytochrome P450, family 27, subfamily b, polypeptide 1 |
Alias Symbols | 1alpha(OH)ase;25(OH)D 1alpha-hydroxylase;25-hydroxyvitamin D(3) 1-alpha-hydroxylase;25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial;25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial precursor (25-OHD-1 alpha-hydroxylase) (25-hydroxyvitamin D3 1-alpha-hydroxylase) (VD3 1A hydroxylase) (P450C1 alpha) (P450VD1-alpha);25-hydroxyvitamin D3 1alpha-hydroxylase;25-OHD-1 alpha-hydroxylase;calcidiol 1-monooxygenase;Cp2;Cp2b;Cyp;Cyp1;Cyp27;Cyp27b;Cyp4;Cyp40;cytochrome p450 27B1;cytochrome P450 subfamily XXVIIB polypeptide 1;cytochrome P450, 27b1;cytochrome P450, 40 (25-hydroxyvitamin D3 1 alpha-hydroxylase);cytochrome P450C1 alpha;cytochrome P450VD1-alpha;P450c;P450c1;P450VD1alpha;Pd;Pddr;Vd;VD3 1A hydroxylase;Vdd;Vdd1;Vddr;VddrI;Vdr. |
NCBI Gene Id | 13115 |
Protein Name | 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial |
Description of Target | Catalyzes the conversion of 25-hydroxyvitamin D3 (25(OH)D3) to 1-alpha,25-dihydroxyvitamin D3 (1alpha,25(OH)(2)D3), and of 24,25-dihydroxyvitamin D3 (24,25(OH)(2)D3) to 1-alpha,24,25-trihydroxyvitamin D3 (1alpha,24,25(OH)(3)D3). Is also active with 25-hydroxy-24-oxo-vitamin D3. Plays an important role in normal bone growth, calcium metabolism, and tissue differentiation. |
Uniprot ID | O35084 |
Protein Accession # | NP_034139.2 |
Nucleotide Accession # | NM_010009.2 |
Protein Size (# AA) | 507 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
Write Your Own Review
We found other products you might like!