Catalog No: OKCD02483
Size:96WELLS
Price: $700.00
SKU
OKCD02483
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Datasheets/ManualsPrintable datasheet for CYP27B1 ELISA Kit (Mouse) (OKCD02483)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 27B1 (CYP27B1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 27B1 (CYP27B1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 27B1 (CYP27B1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cytochrome P450 27B1 (CYP27B1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.625-40ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome P450 27B1 (CYP27B1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome P450 27B1 (CYP27B1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CYP27B1 Microplate96 Wells (12 x 8 Well strips)
CYP27B1 Lyophilized Standard2
100X Biotinylated CYP27B1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity0.235 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cytochrome P450 27B1 (CYP27B1). No significant cross-reactivity or interference between Cytochrome P450 27B1 (CYP27B1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolCyp27b1
Gene Full Namecytochrome P450, family 27, subfamily b, polypeptide 1
Alias Symbols1alpha(OH)ase;25(OH)D 1alpha-hydroxylase;25-hydroxyvitamin D(3) 1-alpha-hydroxylase;25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial;25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial precursor (25-OHD-1 alpha-hydroxylase) (25-hydroxyvitamin D3 1-alpha-hydroxylase) (VD3 1A hydroxylase) (P450C1 alpha) (P450VD1-alpha);25-hydroxyvitamin D3 1alpha-hydroxylase;25-OHD-1 alpha-hydroxylase;calcidiol 1-monooxygenase;Cp2;Cp2b;Cyp;Cyp1;Cyp27;Cyp27b;Cyp4;Cyp40;cytochrome p450 27B1;cytochrome P450 subfamily XXVIIB polypeptide 1;cytochrome P450, 27b1;cytochrome P450, 40 (25-hydroxyvitamin D3 1 alpha-hydroxylase);cytochrome P450C1 alpha;cytochrome P450VD1-alpha;P450c;P450c1;P450VD1alpha;Pd;Pddr;Vd;VD3 1A hydroxylase;Vdd;Vdd1;Vddr;VddrI;Vdr.
NCBI Gene Id13115
Protein Name25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
Description of TargetCatalyzes the conversion of 25-hydroxyvitamin D3 (25(OH)D3) to 1-alpha,25-dihydroxyvitamin D3 (1alpha,25(OH)(2)D3), and of 24,25-dihydroxyvitamin D3 (24,25(OH)(2)D3) to 1-alpha,24,25-trihydroxyvitamin D3 (1alpha,24,25(OH)(3)D3). Is also active with 25-hydroxy-24-oxo-vitamin D3. Plays an important role in normal bone growth, calcium metabolism, and tissue differentiation.
Uniprot IDO35084
Protein Accession #NP_034139.2
Nucleotide Accession #NM_010009.2
Protein Size (# AA)507
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