CYP27B1 ELISA Kit (Human) : 96 Wells (OKEH02077)

Catalog No: OKEH02077

Size:96T

Human CYP27B1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology CYP27B1 ELISA Kit (Human) (OKEH02077) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CYP27B1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CYP27B1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CYP27B1 captured in well.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.3% (n = 20)
  Mean Inter-assay CV%: < 7.1% (n = 20)
• ELISA Kit Component:
  • CYP27B1 Microplate: 96 Wells (12 x 8 Well strips)
  • CYP27B1 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated GLP1R Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.33 ng/mL
• Specificity: Natural and recombinant Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CYP27B1
• Gene Full Name: cytochrome P450 family 27 subfamily B member 1
• Alias Symbols: 1alpha(OH)ase;25 hydroxyvitamin D3-1-alpha hydroxylase;25-hydroxyvitamin D(3) 1-alpha-hydroxylase;25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial;25-OHD-1 alpha-hydroxylase;calcidiol 1-monooxygenase;CP2B;CYP1;CYP1alpha;CYP27B;cytochrome p450 27B1;cytochrome P450 subfamily XXVIIB polypeptide 1;cytochrome P450, family 27, subfamily B, polypeptide 1;cytochrome P450C1 alpha;cytochrome P450VD1-alpha;P450c1;PDDR;VD3 1A hydroxylase;VDD1;VDDR;VDDRI;VDR.
• NCBI Gene Id: 1594
• Protein Name: O15528
• Description of Target: 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
• Uniprot ID: https://www.uniprot.org/uniprot/O15528
• Protein Accession #: NP_000776.1
• Nucleotide Accession #: NM_000785.3
• Protein Size (# AA): 508
• Molecular Weight: 57 kDa