CYP1A2 ELISA Kit (Human) (OKCD02481)

Catalog No: OKCD02481

Size:96WELLS

Human CYP1A2 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 1A2 (CYP1A2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 1A2 (CYP1A2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 1A2 (CYP1A2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cytochrome P450 1A2 (CYP1A2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.625-40ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome P450 1A2 (CYP1A2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome P450 1A2 (CYP1A2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CYP1A2 Microplate: 96 Wells (12 x 8 Well strips)
  • CYP1A2 Lyophilized Standard: 2
  • 100X Biotinylated CYP1A2 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates, cell culture supernatant and other biological fluids
• Sensitivity: 0.264 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cytochrome P450 1A2 (CYP1A2). No significant cross-reactivity or interference between Cytochrome P450 1A2 (CYP1A2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CYP1A2
• Gene Full Name: cytochrome P450 family 1 subfamily A member 2
• Alias Symbols: aryl hydrocarbon hydroxylase;cholesterol 25-hydroxylase;CP12;CYPIA2;cytochrome P(3)450;cytochrome P450 1A2;cytochrome P450 4;cytochrome P450, family 1, subfamily A, polypeptide 2;cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 2;cytochrome P450-P3;dioxin-inducible P3-450;flavoprotein-linked monooxygenase;hydroperoxy icosatetraenoate dehydratase;microsomal monooxygenase;P3-450;P450 form 4;P450(PA);xenobiotic monooxygenase.
• NCBI Gene Id: 1544
• Protein Name: Cytochrome P450 1A2
• Description of Target: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. Also acts in the metabolism of aflatoxin B1 and acetaminophen. Participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. Catalizes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin.
• Uniprot ID: P05177
• Protein Accession #: NP_000752.2
• Nucleotide Accession #: NM_000761.4
• Protein Size (# AA): 515