Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKEH01490
Size:96T
Price: $525.00
SKU
OKEH01490
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.

CYP19A1 ELISA Kit (Human) : 96 Wells (OKEH01490)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH01490
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CYP19A1 ELISA Kit (Human) (OKEH01490) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CYP19A1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CYP19A1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CYP19A1 captured in well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 86%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.6% (n = 20)
Mean Inter-assay CV%: < 7.9% (n = 20)
ELISA Kit Component
ComponentAmount
CYP19A1 Microplate96 Wells (12 x 8 Well strips)
CYP19A1 Lyophilized Standard2
100X Biotinylated CYP19A1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity7.8 pg/mL
SpecificityNatural and recombinant Human Aromatase
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCYP19A1
Gene Full Namecytochrome P450 family 19 subfamily A member 1
Alias SymbolsARO;ARO1;aromatase;CPV1;CYAR;CYP19;CYPXIX;cytochrome P450 19A1;cytochrome P450, family 19, subfamily A, polypeptide 1;cytochrome P450, subfamily XIX (aromatization of androgens);cytochrome P-450AROM;estrogen synthase;estrogen synthetase;flavoprotein-linked monooxygenase;microsomal monooxygenase;P-450AROM.
NCBI Gene Id1588
Protein NameP11511
Description of TargetAromatase
Uniprot IDhttps://www.uniprot.org/uniprot/P11511
Protein Accession #NP_000094.2
Nucleotide Accession #NM_000103.3
Protein Size (# AA)503
Molecular Weight58 kDa
Write Your Own Review
You're reviewing:CYP19A1 ELISA Kit (Human) : 96 Wells (OKEH01490)
Your Rating
We found other products you might like!