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Catalog No: OKEH03438
Size:96T
Price: $525.00
SKU
OKEH03438
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CYP17A1 ELISA Kit (Rat) (OKEH03438) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cyp17a1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Cyp17a1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cyp17a1 captured in well.
ELISA Kit Range0.625-40ng/mL
ELISA Kit Component
ComponentAmount
Anti-Cyp17a1 Microplate96 Wells (12 x 8 Well strips)
Cyp17a1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Cyp17a1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.312 ng/mL
SpecificityNatural and recombinant Rat Steroid 17-alpha-hydroxylase/17,20 lyase
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCyp17a1
Gene Full Namecytochrome P450, family 17, subfamily a, polypeptide 1
Alias Symbols17-alpha-hydroxyprogesterone aldolase;Cyp17;CYPXVII;cytochrome P450 17A1;cytochrome P450, subfamily 17;Cytochrome P450, subfamily XVII;cytochrome P450c17;cytochrome P450-C17;P45017alpha;P450c17;P450-C17;steroid 17-alpha-hydroxylase/17,20 lyase;steroid 17-alpha-monooxygenase.
NCBI Gene Id25146
Protein NameP11715
Description of TargetSteroid 17-alpha-hydroxylase/17,20 lyase
Uniprot IDhttps://www.uniprot.org/uniprot/P11715
Protein Accession #NP_036885.1
Nucleotide Accession #NM_012753.2
Protein Size (# AA)507
Molecular Weight57 kDa
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