Catalog No: OKCD01247 (Formerly GWB-KBBRH1)
Size:96WELLS
Price: $725.00
SKU
OKCD01247
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Datasheets/ManualsPrintable datasheet for CYGB ELISA Kit (Rat) (OKCD01247)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytoglobin (CYGB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytoglobin (CYGB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytoglobin (CYGB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cytoglobin (CYGB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.78-50ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytoglobin (CYGB) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytoglobin (CYGB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Cygb Microplate96 Wells (12 x 8 Well Strips)
Cygb Lyophilized Standard2 x 50 ng
100X Biotinylated Cygb Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.33 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cytoglobin (CYGB). No significant cross-reactivity or interference between Cytoglobin (CYGB) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCygb
Gene Full Namecytoglobin
Alias Symbolscytoglobin;Glnf;globin f;HGb;histoglobin;Staap;Stap;stellate cell activation-associated protein.
NCBI Gene Id170520
Protein NameCytoglobin
Description of TargetMay have a protective function during conditions of oxidative stress. May be involved in intracellular oxygen storage or transfer. Plays a role in the development of liver fibrosis. Has a peroxidase activity.
Uniprot IDQ921A4
Protein Accession #NP_599030
Nucleotide Accession #NM_134268.4
Protein Size (# AA)190
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