CXCR3 ELISA Kit (Rat) (OKCD02369)

Catalog No: OKCD02369

Size:96WELLS

Rat CXCR3 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-X-C-Motif Receptor 3 (CXCR3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chemokine C-X-C-Motif Receptor 3 (CXCR3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine C-X-C-Motif Receptor 3 (CXCR3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Chemokine C-X-C-Motif Receptor 3 (CXCR3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemokine C-X-C-Motif Receptor 3 (CXCR3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemokine C-X-C-Motif Receptor 3 (CXCR3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CXCR3 Microplate: 96 Wells (12 x 8 Well strips)
  • CXCR3 Lyophilized Standard: 2
  • 100X Biotinylated CXCR3 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: 0.059 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Chemokine C-X-C-Motif Receptor 3 (CXCR3). No significant cross-reactivity or interference between Chemokine C-X-C-Motif Receptor 3 (CXCR3) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Cxcr3
• Gene Full Name: C-X-C motif chemokine receptor 3
• Alias Symbols: chemokine (C-X-C motif) receptor 3;C-X-C chemokine receptor type 3;CXCR-3;CXC-R3;G protein-coupled receptor 9;Gpr9;interferon-inducible protein 10 receptor;IP-10 receptor.
• NCBI Gene Id: 84475
• Protein Name: C-X-C chemokine receptor type 3
• Description of Target: Receptor for the C-X-C chemokine CXCL9, CXCL10 and CXCL11 and mediates the proliferation, survival and angiogenic activity of mesangial cells through a heterotrimeric G-protein signaling pathway. Binds to CCL21. Probably promotes cell chemotaxis response (By similarity).
• Uniprot ID: Q9JII9
• Protein Accession #: NP_445867.1
• Nucleotide Accession #: NM_053415.1
• Protein Size (# AA): 367