Catalog No: OKCD02479
Size:96WELLS
Price: $625.00
SKU
OKCD02479
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Datasheets/ManualsPrintable datasheet for CXCR3 ELISA Kit (Mouse) (OKCD02479)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
79-101%80-93%78-93%81-101%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-X-C-Motif Receptor 3 (CXCR3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chemokine C-X-C-Motif Receptor 3 (CXCR3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine C-X-C-Motif Receptor 3 (CXCR3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Chemokine C-X-C-Motif Receptor 3 (CXCR3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range78-5,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
85-9289
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemokine C-X-C-Motif Receptor 3 (CXCR3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemokine C-X-C-Motif Receptor 3 (CXCR3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CXCR3 Microplate96 Wells (12 x 8 Well strips)
CXCR3 Lyophilized Standard2
100X Biotinylated CXCR3 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity0.125 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Chemokine C-X-C-Motif Receptor 3 (CXCR3). No significant cross-reactivity or interference between Chemokine C-X-C-Motif Receptor 3 (CXCR3) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolCxcr3
Gene Full Namechemokine (C-X-C motif) receptor 3
Alias SymbolsCd183;Cmka;Cmkar3;C-X-C chemokine receptor type 3;Cxcr;interferon-inducible protein 10 receptor;IP-10 receptor.
NCBI Gene Id12766
Protein NameC-X-C chemokine receptor type 3
Description of TargetReceptor for the C-X-C chemokine CXCL9, CXCL10 and CXCL11 and mediates the proliferation, survival and angiogenic activity of mesangial cells through a heterotrimeric G-protein signaling pathway. Probably promotes cell chemotaxis response (By similarity). Binds to CCL21.
Uniprot IDO88410
Protein Accession #NP_034040.1
Nucleotide Accession #NM_009910.3
Protein Size (# AA)367
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