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Catalog No: OKEH00032
Size:96T
Price: $675.00
SKU
OKEH00032
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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CXCR1 ELISA Kit (Human) : 96 Wells (OKEH00032)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CXCR1 ELISA Kit (Human) (OKEH00032) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CXCR1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CXCR1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CXCR1 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.9% (n = 20)
Mean Inter-assay CV%: < 7.2% (n = 20)
ELISA Kit Component
ComponentAmount
CXCR1 Microplate96 Wells (12 x 8 Well strips)
CXCR1 Lyophilized Standard2
100X Biotinylated CXCR1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.045 ng/mL
SpecificityNatural and recombinant Human C-X-C chemokine receptor type 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCXCR1
Gene Full NameC-X-C motif chemokine receptor 1
Alias SymbolsC-C;C-C-CKR-1;CD128;CD181;CDw128a;chemokine (C-X-C motif) receptor 1;CKR-1;CMKAR1;C-X-C chemokine receptor type 1;CXCR-1;CXC-R1;high affinity interleukin-8 receptor A;IL-8 receptor type 1;IL-8R A;IL8R1;IL8RA;IL8RBA;interleukin 8 receptor, alpha;interleukin-8 receptor type 1;interleukin-8 receptor type A.
NCBI Gene Id3577
Protein NameP25024
Description of TargetC-X-C chemokine receptor type 1
Uniprot IDhttps://www.uniprot.org/uniprot/P25024
Protein Accession #NP_000625
Nucleotide Accession #NM_000634
Protein Size (# AA)350
Molecular Weight40 kDa
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