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CXCL8 ELISA Kit (Human) : 96 Wells (OKAA00042_96W)

  • Catalog#: OKAA00042_96W
  • 2 weeks

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96 Wells
$230.00
In Stock
Application:
ELISA
Gene Symbol:
CXCL8
Official Gene Full Name:
C-X-C motif chemokine ligand 8
NCBI Gene Id:
3576
Alias Symbols:
IL8, NAF, GCP1, LECT, LUCT, NAP1, GCP-1, LYNAP, MDNCF, MONAP, NAP-1
Swissprot Id:
P10145
Protein Accession #:
NP_000575.1
Nucleotide Accession #:
NM_000584.3
Species Reactivity:
Human
Sample Type:
cell culture, supernates, serum, and plasma
Sensitivity:
4 pg/ml
Kit Reproducibility:
The coefficient of variation of both intra-assay and inter-assay were less than 10%.
Kit Component:
Components
2 vials human IL-8 Standard lyophilized, 2000 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-human IL-8 polyclonal antibody
2 vials Streptavidin-HRP solution
1 bottle Standard /sample Diluent
1 bottle Biotin-Conjugate antibody Diluent
1 bottle Streptavidin-HRP Diluent
1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
1 vial Substrate Solution
1 vial Stop Solution
2 vials mouse IL-6 Standard lyophilized, 2000 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-mouse IL-6 antibody
Description of Target:
The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q.
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CXCL8.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CXCL8.
Predicted Homology Based on Immunogen Sequence:
Human
Datasheets / Downloads:
OKAA00042_96W
Specificity:
This assay recognizes both natural and recombinant human IL-2. The factors listed below were prepared at 100ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.

Recombinant human: IL-2 sRa, IL-2 Ra, IL-2 Rg, IL-3, IL-4, IL-6, IL-7, TGF-b2, TNF-a, TNF-b

Recombinant mouse: IL-2, IL-1, IL-2, IL-4, IL-5, IL-6 ,

Other proteins: bovine FGF acidic, bovine FGF basic, human PDGF, porcine PDGF, human TGF-b1, porcine TGF-b1
Assay Info:
Assay Method: Quantitative Sandwich ELISA
Additional Information:
Sample Type: cell culture supernates, serum, and plasma
:::
Reagents: REAGENTS
1. Aluminum pouches with a Microwell Plate coated with monoclonal antibody to human IL-8 (8x12)
2. 2 vials human IL-8 Standard lyophilized, 2000 pg/ml upon reconstitution
3. 2 vials concentrated Biotin-Conjugate anti-human IL-8 polyclonal antibody
4. 2 vials Streptavidin-HRP solution,
5. 1 bottle Standard /sample Diluent
6. 1 bottle Biotin-Conjugate antibody Diluent
7. 1 bottle Streptavidin-HRP Diluent
8. 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
9. 1 vial Substrate Solution
10. 1 vial Stop Solution
11. 3 pieces Adhesive Films
12. package insert
Protein Name:
interleukin-8
Protocol:
Tips Information:
 
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