CXCL4/PF4 ELISA Kit (Mouse) (OKBB00369)

Catalog No: OKBB00369

Size:96 Wells

Mouse CXCL4/PF4 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's mouse CXCL4 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CXCL4 has been precoated onto 96-well plates. Standards(E.coli, V30-S105) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse CXCL4 amount of sample captured in plate.
• ELISA Kit Range: 15.6 pg/ml - 1,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	84.2	266	524	92.1	285	519
  SD	4.97	16.49	25.2	6.63	21.09	31.7
  CV%	5.9	6.2	4.8	7.2	7.4	6.1

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Mouse CXCL4 Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Mouse CXCL4 Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Mouse CXCL4 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum and plasma (heparin, EDTA)
• Sensitivity: <1 pg/ml
• Specificity: Natural and recombinant mouse CXCL4
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: V30-S105
• Description: For quantitative detection of mouse CXCL4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: PF4
• Gene Full Name: platelet factor 4
• Alias Symbols: C X C motif chemokine 4, C-X-C motif chemokine 4, Chemokine (C X C motif) ligand 4, Chemokine (CXC motif) ligand 4, Chemokine, CXC motif, ligand 4, CXCL 4, CXCL4, Iroplact, MGC138298, Oncostatin A, Oncostatin-A, OncostatinA, PF 4, PF-4, PF4, Pf4a, Platelet factor 4, PLF4_HUMAN, RATPF4A, SCYB 4, SCYB4, short form, Small inducible cytokine subfamily B, member 4, Small inducible cytokine subfamily member 4
• NCBI Gene Id: 56744
• Protein Name: Platelet factor 4
• Description of Target: Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). The PF4 gene was localized on map to 4q12-q13. Chemokines play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.
• Uniprot ID: Q9Z126
• Protein Accession #: NP_064316.1
• Nucleotide Accession #: NM_019932.4