Catalog No: OKEH04515
Size:96T
Price: $425.00
SKU
OKEH04515
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Cxcl2 ELISA Kit (Mouse) (OKEH04515) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cxcl2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Cxcl2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cxcl2 captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
Anti-Cxcl2 Microplate96 Wells (12 x 8 Well Strips)
Cxcl2 Lyophilized Standard2
Sample Diluent1 x 20 mL
Cxcl2 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample Typeserum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids
Sensitivity0.166 ng/mL
SpecificityNatural and recombinant Mouse C-X-C motif chemokine 2
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolCxcl2
Gene Full Namechemokine (C-X-C motif) ligand 2
Alias SymbolsCINC;CINC-2a;C-X-C motif chemokine 2;Gr;Gro2;GROb;macrophage inflammatory protein 2;Mgsa;Mgsa-b;Mi;MIP;Mip2;MIP-2;MIP-2a;Scy;Scyb;Scyb2;small inducible cytokine subfamily, member 2.
NCBI Gene Id20310
Protein NameP10889
Description of TargetC-X-C motif chemokine 2
Uniprot IDhttps://www.uniprot.org/uniprot/P10889
Protein Accession #NP_033166.1
Nucleotide Accession #NM_009140.2
Protein Size (# AA)100
Molecular Weight10 KDa
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