CXCL13 ELISA Kit (Human) (OKEH04027)

Catalog No: OKEH04027

Size:96T

Human CXCL13 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology CXCL13 ELISA Kit (Human) (OKEH04027) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CXCL13 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CXCL13 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CXCL13 captured in the well.
• ELISA Kit Range: 7.8-500pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 5.9% (n = 20)
  Mean Inter-assay CV%: <= 8.3% (n = 20)
• ELISA Kit Component:
  • Anti-CXCL13 Microplate: 96 Wells (12 x 8 Well Strips)
  • CXCL13 Lyophilized Standard: 2 x 2 ng
  • Sample Diluent: 1 x 20 mL
  • CXCL13 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 3.2 pg/mL
• Specificity: Natural and recombinant Human C-X-C motif chemokine 13
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CXCL13
• Gene Full Name: C-X-C motif chemokine ligand 13
• Alias Symbols: ANGIE;ANGIE2;b cell-attracting chemokine 1;b lymphocyte chemoattractant;BCA1;BCA-1;B-cell chemoattractant;B-cell-attracting chemokine 1;B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1);BLC;BLR1L;B-lymphocyte chemoattractant;chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant);CXC chemokine BLC;C-X-C motif chemokine 13;SCYB13;small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cell chemoattractant);small-inducible cytokine B13.
• NCBI Gene Id: 10563
• Protein Name: O43927
• Description of Target: C-X-C motif chemokine 13
• Uniprot ID: https://www.uniprot.org/uniprot/O43927
• Protein Accession #: NP_006410.1
• Nucleotide Accession #: NM_006419.2
• Protein Size (# AA): 109
• Molecular Weight: 13 kDa