CXCL10/IP-10 ELISA Kit (Mouse) (OKBB00316)

Catalog No: OKBB00316 (Formerly GWB-RYBRS2)

Size:96 Tests

Mouse CXCL10 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's mouse CXCL10 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CXCL10 has been precoated onto 96-well plates. Standards(E.coli, I22-P98) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL10 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse CXCL10 amount of sample captured in plate.
• ELISA Kit Range: 31.2 pg/ml - 2,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  Mean (pg/mL)	112	259	973	125	276	1093
  St. Dev.	5.82	14.5	70.1	12.25	22.4	84.2
  %CV	5.19	5.59	7.20	9.8	8.11	7.70

• ELISA Kit Component:
  • Lyophilized recombinant human CXCL10 standard: 10 ng/tube x 2
  • Anti-human CXCL10a Antibody Well Plate: 96 Wells
  • Sample diluent buffer: 30 mL
  • Biotinylated anti-human CXCL10 antibody: 130 uL, dilution 1:100
  • Antibody diluent buffer: 12 mL
  • Avidin-Biotin-Peroxidase Complex (ABC): 130 uL, dilution 1:100
  • ABC diluent buffer: 12 mL
  • TMB color developing agent: 10 mL
  • TMB stop solution: 10 mL
• Additional Information: Range: 31.2pg/ml-2000pg/ml
  Principle: Aviva’s mouse CXCL10 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse CXCL10 specific-specific polyclonal antibodies were precoated onto 96-well plates. The mouse specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CXCL10 amount of sample captured in plate.
  Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
  experiment using standards and a small number of samples is recommended.
  2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
  3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
  4. Duplicate well assay is recommended for both standard and sample testing.
  5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
  6. Don’t reuse tips and tubes to avoid cross contamination.
  7. To avoid to use the reagents from different batches together.
  8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
  marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
  using.
  Background: Chemokine (C-X-C motif) ligand 10 (CXCL10) or IP-10 is a small cytokine belonging to the CXC chemokine family that is also known as 10 kDa interferon-gamma-induced protein (γ-IP10 or IP-10). CXCL10 is secreted by several cell types in response to IFN-γ. These cell types include monocytes, endothelial cells and fibroblasts. CXCL10 has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis. It is a potent inhibitor of angiogenesis in vivo. The gene for CXCL10 is located on human chromosome 4 in a cluster among several other CXC chemokines. It is a RAS target gene and is overexpressed in the majority of colorectal cancers. This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3.
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates, serum and plasma (heparin)
• Sensitivity: <1 pg/ml
• Predicted Homology Based on Immunogen Sequence: No detectable cross-reactivity with any other cytokine.
• Reference: 1. Luster et al. Gamma-interferon transcriptionally regulates an early-response gene containing homology to platelet
  proteins. Nature 315: 672-676, 1985.
  2. Dufour et al. IFN-gamma-inducible protein 10 (IP-10; CXCL10) -deficient mice reveal a role for IP-10 in effector T
  cell generation and trafficking. J. Immun. 168:, 2002.
  3. Angiolillo et al. Human interferon-inducible protein 10 is a potent inhibitor of angiogenesis in vivo. J. Exp. Med. 182:
  155-162, 1995.
  4. Angiolillo, A. L.; Sgadari, C.; Taub, D. D.; Liao, F.; Farber, J. M.; Maheshwari, S.; Kleinman, H. K.; Reaman, G. H.;
  Tosato, G. : Human interferon-inducible protein 10 is a potent inhibitor of angiogenesis in vivo. J. Exp. Med. 182:
  155-162, 1995.
  5. O'Donovan et al. Physical mapping of the CXC chemokine locus on human chromosome 4. Cytogenet. Cell Genet.
  84: 39-42, 1999.
  6. Zhang, R.; Zhang, H.; Zhu, W.; Pardee, A. B.; Coffey, R. J., Jr.; Liang, P. : Mob-1, a Ras target gene, is
  overexpressed in colorectal cancer. Oncogene 14: 1607-1610, 1997.
  7. Booth et al. The CXCR3 binding chemokine IP-10/CXCL10: structure and receptor interactions. Biochemistry 41:,
  2002
• Specificity: Natural and recombinant mouse CXCL10
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: I22-P98
• Description: For quantitative detection of mouse CXCL10 in cell culture supernatants, serum and plasma( heparin).

Target Info

• Gene Symbol: CXCL10
• Gene Full Name: chemokine (C-X-C motif) ligand 10
• Alias Symbols: 10 kDa interferon gamma-induced protein, C7, Chemokine (C X C motif) ligand 10, Chemokine CXC motif ligand 10, Crg 2, CRG2, CXCL10, CXCL10(1-73), CXL10_HUMAN, Gamma IP10, Gamma-IP10, gIP 10, GIP10, IFI10, INP 10, Interferon activated gene 10, Interferon gamma induced cell line, Interferon inducible cytokine IP10, IP 10, IP-10, MOB1, SCYB10, Small inducible cytokine B10, Small inducible cytokine B10 precursor, Small inducible cytokine subfamily B (Cys X Cys) member 10, Small inducible cytokine subfamily B CXC member 10, Small-inducible cytokine B10
• NCBI Gene Id: 15945
• Protein Name: C-X-C motif chemokine 10
• Description of Target: Chemokine (C-X-C motif) ligand 10 (CXCL10) or IP-10 is a small cytokine belonging to the CXC chemokine family that is also known as 10 kDa interferon-gamma-induced protein (γ-IP10 or IP-10). CXCL10 is secreted by several cell types in response to IFN-γ. These cell types include monocytes, endothelial cells and fibroblasts. CXCL10 has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis.It is a potent inhibitor of angiogenesis in vivo. The gene for CXCL10 is located on human chromosome 4 in a cluster among several other CXC chemokines. It is a RAS target gene and is overexpressed in the majority of colorectal cancers.This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3.
• Uniprot ID: P17515
• Protein Accession #: NP_067249.1
• Nucleotide Accession #: NM_021274.2