website statistics
Account Login 

Aviva Systems Biology office will be closed for Thanksgiving - Thursday 11/27/2014 and Friday 11/28/2014.
Please go here for more info.

Now Offering Over 85,203 Antibodies & 34,301 Antigens!

Print Page
50ug
$555.00
In Stock

Cxcl1 Antibody (OASA08385)

Aviva Systems Biology is a leading manufacturer of antibodies, proteins, peptides, and accessory reagents. Aviva’s catalog is filled with many unique products. If you need assistance at any time in your searches, please email us at info@avivasysbio.com.

Aviva has generated over 5,000 product categories to assist you when browsing our catalog. Please select the related products tab below to view other relevant reagents.

Please review the product specifications below.
Description of Target:
RABBIT ANTI MOUSE CXCL1:Biotin
Gene Symbol:
Cxcl1
NCBI Gene Id:
14825
Alias Symbols:
KC; Fsp; N51; gro; Gro1; Mgsa; Scyb1; Cxcl1
Tissue Tool:
Find tissues and cell lines supported to express Cxcl1.
Protein Accession #:
NP_032202.1
Swissprot Id:
P12850
Host:
Rabbit
Protein Name:
Growth-regulated alpha protein
Protein Size (# AA):
96
Specificity:
CXCL1
Application:
ELISA, WB
Conjugation:
Biotin
Immunogen:
The immunogen for anti-Cxcl1 antibody: recombinant mouse CXCL1
Product Format:
Purified IgG conjugated to Biotin - lyophilised
Isotype:
Polyclonal IgG
Predicted Homology Based on Immunogen Sequence:
Mouse
Species Reactivity:
Mouse
Datasheets / Downloads:
Printable datasheet for
anti-Cxcl1 antibody
- OASA08385
Applications Info:
ELISA:    0.15ug/ml - 0.30ug/ml
Western Blotting:    0.1ug/ml - 0.2ug/ml
Additional Information:
Note: Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Aviva Systems Biology recommend that the vial is gently mixed after reconstitution.
The addition of 0.09% sodium azide is recommende
:::
Preservative Stabilisers: None present

Antiserum Preparation: Antisera to mouse CXCL1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
:::
Approx Protein Conc: IgG concentration 50ug/ml

Buffer Solutions: Phosphate buffered saline
Reconstitution and Storage:
Reconstitution: Reconstitute with 1.0ml sterile PBS containing 0.1% Bovine Serum Albumin
Storage: Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Product Protocol: Cxcl1 antibody used to evaluate decidual stromal cell response to paracrine signals from the trophoblast: amplification of immune and angiogenic modulators (OASA08385)

Specificity: CXCL1
Product Page: Cxcl1 antibody (OASA08385)
Antibody Pair Available: OASA08384 Capture OASA08385 Detection
Experiment Type: Protein Expression ELISA evaluations

Protocol:
A.P. Hess et al. chose two of the most highly upregulated genes (CXCL1 [GRO1] and IL8) for protein validation by ELISA. For both assays, cell culture supernatant (~1000 μl) was removed from the sample wells, at t = 0 of treatment and at the appropriate time interval (3 or 12 h), and diluted in the appropriate diluents for each respective assay. The CXCL1 ELISA was performed with a monoclonal anti-human CXCL1 antibody (R&D Systems, Minneapolis, MN). One hundred microliters per well of primary antibody (at a concentration of 4 μg/ml) was coated overnight onto 96-well Maxisorp plates (Nalge Nunc International, Rochester, NY). Plates were washed with wash buffer (1× PBS + 0.05% v/v Tween-20) and then blocked for 1 h at room temperature with blocking buffer (1× PBS + 1% w/v BSA, 5% w/v sucrose, and 0.05% w/v NaN3). Serial dilutions of recombinant CXCL1 (R&D Systems), ranging from 60 pg/ml to 4 ng/ml, were used as the standard. One hundred microliters of standard or appropriately diluted culture supernatant was added to each well and incubated for 2 h at room temperature. After washing, 100 μl of biotinylated anti-human CXCL1 at a concentration of 200 ng/ml was used to complete the sandwich assay and was added to each well for 2 h at room temperature. A streptavidin-HRP conjugate was then added to the plate and incubated for 20 min at 25°C. The color was developed with a tetramethylbenzidine substrate solution, and the absorbance was measured at 450 nm.

Interleukin 8 (IL8) was assayed in the cell culture supernatant with the Endogen Human IL8 ELISA Kit (Pierce Biotechnology, Rockford, IL). Serial dilutions of the IL8 standard ranged from 25.6 pg/ml to 1.0 ng/ml.

The mean ± SEM for the n = 3 replicates was calculated. Paired t-test analyses were performed to determine the statistical significance between the TCM and CCM treatments, as well as between t = 0 and the respective treatment conditions (CCM and TCM).

Summary:
1. Baseline levels of CXCL1 in conditioned media at t = 0 were 7.05 ng/ml, which rose to 34.33 ng/ml after 3 h of treatment, compared to 14.82 ng/ml in the control condition. By 12 h of TCM treatment, the protein level increased to 118.40 ng/ml, compared to virtually no change in the control. 2. The increase in CXCL1 was statistically significant at both 3 and 12 h of TCM treatment, compared to CCM, by paired t-test analysis. In addition, the CXCL1 levels were statistically significant in the TCM compared to t = 0 at both 3 and 12 h by paired t-test analysis (data not shown). However, there was no significant difference between the CCM CXCL1 levels and t = 0 (data not shown).

References:
1: Hess AP, Hamilton AE, Talbi S, Dosiou C, Nyegaard M, Nayak N, Genbecev-Krtolica O, Mavrogianis P, Ferrer K, Kruessel J, Fazleabas AT, Fisher SJ, Giudice LC. Decidual stromal cell response to paracrine signals from the trophoblast: amplification of immune and angiogenic modulators. Biol Reprod. 2007 Jan;76(1):102-17. Epub 2006 Oct 4. PubMed PMID: 17021345.

 
X
Ask a Question