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Catalog No: OKEH04503
Size:96T
Price: $425.00
SKU
OKEH04503
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Cx3cl1 ELISA Kit (Mouse) (OKEH04503) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cx3cl1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Cx3cl1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cx3cl1 captured in well.
ELISA Kit Range0.39-25ng/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
Anti-Cx3cl1 Microplate96 Wells (12 x 8 Well Strips)
Cx3cl1 Lyophilized Standard2
Sample Diluent1 x 20 mL
Cx3cl1 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample Typeserum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids
Sensitivity0.195 ng/mL
SpecificityNatural and recombinant Mouse Fractalkine
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCx3cl1
Gene Full Namechemokine (C-X3-C motif) ligand 1
Alias SymbolsAB030188;ABCD-3;AI848747;CX3C;CX3C membrane-anchored chemokine;C-X3-C motif chemokine 1;Cxc3;D8Bwg0439e;FK;fract;fractalkine;neuro;neurotactin;Scyd;Scyd1;small inducible cytokine subfamily D, 1;small-inducible cytokine D1.
NCBI Gene Id20312
Protein NameO35188
Description of TargetFractalkine
Uniprot IDhttps://www.uniprot.org/uniprot/O35188
Protein Accession #NP_033168.2
Nucleotide Accession #NM_009142.3
Protein Size (# AA)395
Molecular Weight42 KDa
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