CUBN ELISA Kit (Human) (OKCD02475)

Catalog No: OKCD02475

Size:96WELLS

Human CUBN ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cubilin (CUBN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cubilin (CUBN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cubilin (CUBN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cubilin (CUBN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cubilin (CUBN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cubilin (CUBN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CUBN Microplate: 96 Wells (12 x 8 Well strips)
  • CUBN Lyophilized Standard: 2
  • 100X Biotinylated CUBN Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: 0.112 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cubilin (CUBN). No significant cross-reactivity or interference between Cubilin (CUBN) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CUBN
• Gene Full Name: cubilin
• Alias Symbols: 460 kDa receptor;cubilin;cubilin (intrinsic factor-cobalamin receptor);cubilin precursor variant 1;cubilin precursor variant 2;cubilin precursor variant 3;gp280;IFCR;IGS;IGS1;intestinal intrinsic factor receptor;Intrinsic factor-cobalamin receptor;intrinsic factor-vitamin B12 receptor;MGA1.
• NCBI Gene Id: 8029
• Protein Name: Cubilin
• Description of Target: Cotransporter which plays a role in lipoprotein, vitamin and iron metabolism, by facilitating their uptake. Binds to ALB, MB, Kappa and lambda-light chains, TF, hemoglobin, GC, SCGB1A1, APOA1, high density lipoprotein, and the GIF-cobalamin complex. The binding of all ligands requires calcium. Serves as important transporter in several absorptive epithelia, including intestine, renal proximal tubules and embryonic yolk sac. Interaction with LRP2 mediates its trafficking throughout vesicles and facilitates the uptake of specific ligands like GC, hemoglobin, ALB, TF and SCGB1A1. Interaction with AMN controls its trafficking to the plasma membrane and facilitates endocytosis of ligands. May play an important role in the development of the peri-implantation embryo through internalization of APOA1 and cholesterol. Binds to LGALS3 at the maternal-fetal interface.
• Uniprot ID: O60494
• Protein Accession #: NP_001072.2
• Nucleotide Accession #: NM_001081.3
• Protein Size (# AA): 3623