CTSL ELISA Kit (Mouse) (OKCD02474)

Catalog No: OKCD02474

Size:96WELLS

Mouse CTSL ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	98-105%	79-98%	88-96%	84-98%
  EDTA Plasma (n=5)	80-94%	91-99%	96-104%	81-103%
  Heparin Plasma (n=5)	91-101%	91-101%	83-99%	82-103%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cathepsin L (CTSL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cathepsin L (CTSL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cathepsin L (CTSL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cathepsin L (CTSL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 62.5-4,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	96-105	102
  EDTA Plasma (n=5)	85-98	90
  Heparin Plasma(n=5)	81-98	92

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cathepsin L (CTSL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cathepsin L (CTSL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CTSL Microplate: 96 Wells (12 x 8 Well strips)
  • CTSL Lyophilized Standard: 2
  • 100X Biotinylated CTSL Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 26.2 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cathepsin L (CTSL). No significant cross-reactivity or interference between Cathepsin L (CTSL) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Ctsl
• Gene Full Name: cathepsin L
• Alias Symbols: 1190035F06Rik;Cat L;Cathepsin L;cathepsin L1;CatL;Ctsl1;fs;major excreted protein;ME;MEP;nkt;p39 cysteine proteinase;procathepsin L.
• NCBI Gene Id: 13039
• Protein Name: Cathepsin L1
• Description of Target: Important for the overall degradation of proteins in lysosomes.
• Uniprot ID: P06797
• Protein Accession #: NP_034114.1
• Nucleotide Accession #: NM_009984.4
• Protein Size (# AA): 334