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Datasheets/Manuals | Printable datasheet for CTNNB1 Antibody (Phospho-Tyr489) (OAAF00398) |
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Predicted Species Reactivity | Human|Monkey|Mouse|Rat |
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Product Format | Liquid |
Clonality | Polyclonal |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human Catenin-beta around the phosphorylation site of Tyr489. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Specificity | Catenin-β (Phospho-Tyr489) antibody detects endogenous levels of Catenin-β only when phosphorylated at tyrosine 489. |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Dilution | The optimal working dilution should be determined by the end user. |
Application Info | WB: 1:500~1:3000; IHC: 1:50~1:100; ELISA: 1:40000 |
Reference | Huelsken J., J. Cell Biol. 127:2061-2069(1994). Zhang C., Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases. Kim J.-S., Cancer Res. 62:2744-2748(2002). |
Gene Symbol | CTNNB1 |
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Gene Full Name | catenin beta 1 |
Alias Symbols | armadillo;Beta-catenin;catenin (cadherin-associated protein), beta 1, 88kDa;catenin beta-1;CTNNB;EVR7;MRD19;NEDSDV. |
NCBI Gene Id | 1499 |
Protein Name | Catenin beta-1 |
Description of Target | Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion, as component of an E-cadherin:catenin adhesion complex. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22647378, PubMed:22699938, PubMed:22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity). |
Uniprot ID | P35222 |
Protein Accession # | NP_001091679.1 |
Molecular Weight | 85 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "CTNNB1 Antibody (Phospho-Tyr489) (OAAF00398)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Monkey|Mouse|Rat".
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How long will it take to receive "CTNNB1 Antibody (Phospho-Tyr489) (OAAF00398)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "CTNNB1 Antibody (Phospho-Tyr489) (OAAF00398)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "CTNNB1 Antibody (Phospho-Tyr489) (OAAF00398)"?
This target may also be called "armadillo;Beta-catenin;catenin (cadherin-associated protein), beta 1, 88kDa;catenin beta-1;CTNNB;EVR7;MRD19;NEDSDV." in publications.
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What is the shipping cost for "CTNNB1 Antibody (Phospho-Tyr489) (OAAF00398)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "CTNNB1 Antibody (Phospho-Tyr489) (OAAF00398)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "CTNNB1 Antibody (Phospho-Tyr489) (OAAF00398)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "85 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "CTNNB1 Antibody (Phospho-Tyr489) (OAAF00398)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "CTNNB1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "CTNNB1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "CTNNB1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "CTNNB1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "CTNNB1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "CTNNB1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.