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Catalog No: OKEH03508
Size:96T
Price: $525.00
SKU
OKEH03508
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Ctcf ELISA Kit (Mouse) (OKEH03508) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Mouse Ctcf antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Ctcf and incubated. The Ctcf found in the sample or standards competes with the biotinylated Ctcf for limited binding sites on the immobilized anti-Mouse Ctcf antibody. Excess unbound biotinylated Ctcf and sample or standard Ctcf is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Ctcf captured in the well and inversely proportional to the amount of Ctcf which was contained in the sample or standard.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 7.2% (n = 20)
Mean Inter-assay CV%: <= 9.4% (n = 20)
ELISA Kit Component
ComponentAmount
Ctcf Microplate96 Wells (12 x 8 Well strips)
Ctcf Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Ctcf-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity39.3 pg/mL
SpecificityNatural and recombinant Mouse Transcriptional repressor CTCF
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolCtcf
Gene Full NameCCCTC-binding factor
Alias Symbols11-zinc finger protein;AW108038;CCCTC-binding factor;CTCFL paralog;transcriptional repressor CTCF.
NCBI Gene Id13018
Protein NameQ61164
Description of TargetTranscriptional repressor CTCF
Uniprot IDhttps://www.uniprot.org/uniprot/Q61164
Protein Accession #NP_851839.1
Nucleotide Accession #NM_181322.3
Protein Size (# AA)736
Molecular Weight84 kDa
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