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Catalog No: OKCD00970 (Formerly GWB-KBBZQ3)
Size:96WELLS
Price: $675.00
SKU
OKCD00970
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Datasheets/ManualsPrintable datasheet for CTBP2 ELISA Kit (Human) (OKCD00970)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-Terminal Binding Protein 2 (CTBP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to C-Terminal Binding Protein 2 (CTBP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C-Terminal Binding Protein 2 (CTBP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of C-Terminal Binding Protein 2 (CTBP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C-Terminal Binding Protein 2 (CTBP2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C-Terminal Binding Protein 2 (CTBP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CTBP2 Microplate96 Wells (12 x 8 Well Strips)
CTBP2 Lyophilized Standard2 x 20 ng
100X Biotinylated CTBP2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.125 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of C-Terminal Binding Protein 2 (CTBP2). No significant cross-reactivity or interference between C-Terminal Binding Protein 2 (CTBP2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCTBP2
Gene Full NameC-terminal binding protein 2
Alias SymbolsC-terminal-binding protein 2;ribeye.
NCBI Gene Id1488
Protein NameC-terminal-binding protein 2
Description of TargetCorepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation (By similarity).
Uniprot IDP56545
Protein Accession #NP_001077383
Nucleotide Accession #NM_001083914.2
Protein Size (# AA)445
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