CSTA ELISA Kit (Rat) (OKCD02357)

Catalog No: OKCD02357

Size:96WELLS

Rat CSTA ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	80-93%	94-102%	87-94%	98-105%
  EDTA Plasma (n=5)	97-105%	80-96%	90-104%	97-105%
  Heparin Plasma (n=5)	87-102%	83-101%	85-97%	94-103%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cystatin A (CSTA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cystatin A (CSTA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cystatin A (CSTA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cystatin A (CSTA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	88-102	94
  EDTA Plasma (n=5)	84-102	97
  Heparin Plasma(n=5)	79-101	93

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cystatin A (CSTA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cystatin A (CSTA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CSTA Microplate: 96 Wells (12 x 8 Well strips)
  • CSTA Lyophilized Standard: 2
  • 100X Biotinylated CSTA Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernatant and other biological fluids
• Sensitivity: 0.113 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cystatin A (CSTA). No significant cross-reactivity or interference between Cystatin A (CSTA) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Stfa3
• Gene Full Name: stefin A3
• Alias Symbols: Csta;cystatin-A;cystatin-alpha;epidermal stefin;epidermal thiol proteinase inhibitor.
• NCBI Gene Id: 288075
• Protein Name: Cystatin-A
• Description of Target: This is an intracellular thiol proteinase inhibitor.
• Uniprot ID: P01039
• Protein Accession #: NP_001099347.1
• Nucleotide Accession #: NM_001105877.1
• Protein Size (# AA): 103