CST2 ELISA Kit (Human) (OKCD00563)

Catalog No: OKCD00563 (Formerly GWB-KBBBA5)

Size:96WELLS

Human CST2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cystatin 2 (CST2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cystatin 2 (CST2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cystatin 2 (CST2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cystatin 2 (CST2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cystatin 2 (CST2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cystatin 2 (CST2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CST2 Microplate: 96 Wells (12 x 8 Well Strips)
  • CST2 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated CST2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 0.068 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cystatin 2 (CST2). No significant cross-reactivity or interference between Cystatin 2 (CST2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: CST2
• Gene Full Name: cystatin SA
• Alias Symbols: cystatin 2;cystatin S5;Cystatin-2;Cystatin-S5;cystatin-SA;cysteine-proteinase inhibitor;salivary cysteine (thiol) protease inhibitor.
• NCBI Gene Id: 1470
• Protein Name: P09228
• Description of Target: Cystatin-SA
• Uniprot ID: https://www.uniprot.org/uniprot/P09228
• Protein Accession #: NP_001313
• Nucleotide Accession #: NM_001322.2
• Protein Size (# AA): 141