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Catalog No: OKCD00838 (Formerly GWB-KBBMN5)
Size:96WELLS
Price: $650.00
SKU
OKCD00838
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Datasheets/ManualsPrintable datasheet for CSF2RB ELISA Kit (Human) (OKCD00838)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Colony Stimulating Factor 2 Receptor Beta (CSF2Rb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Colony Stimulating Factor 2 Receptor Beta (CSF2Rb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Colony Stimulating Factor 2 Receptor Beta (CSF2Rb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Colony Stimulating Factor 2 Receptor Beta (CSF2Rb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Colony Stimulating Factor 2 Receptor Beta (CSF2Rb) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Colony Stimulating Factor 2 Receptor Beta (CSF2Rb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CSF2RB Microplate96 Wells (12 x 8 Well Strips)
CSF2RB Lyophilized Standard2 x 20 ng
100X Biotinylated CSF2RB Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.113 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Colony Stimulating Factor 2 Receptor Beta (CSF2Rb). No significant cross-reactivity or interference between Colony Stimulating Factor 2 Receptor Beta (CSF2Rb) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCSF2RB
Gene Full Namecolony stimulating factor 2 receptor subunit beta
Alias Symbolsbeta common cytokine receptor;beta-GM-CSF receptor;betaGMR;CD131;CDw131;colony stimulating factor 2 receptor beta common subunit;colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage);colony-stimulating factor-2 receptor, beta, low-affinity;cytokine receptor common subunit beta;GM-CSF/IL-3/IL-5 receptor common beta subunit;GM-CSF/IL-3/IL-5 receptor common beta-chain;IL3RB;IL5RB;interleukin 3 receptor/granulocyte-macrophage colony stimulating factor 3 receptor, beta (high affinity);SMDP5.
NCBI Gene Id1439
Protein NameCytokine receptor common subunit beta
Description of TargetHigh affinity receptor for interleukin-3, interleukin-5 and granulocyte-macrophage colony-stimulating factor.
Uniprot IDP32927
Protein Accession #NP_000386
Nucleotide Accession #NM_000395.2
Protein Size (# AA)897
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