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CSF2 ELISA Kit (Human) : 96 Wells (OKAA00005_96W)

  • Catalog#: OKAA00005_96W
  • 2 weeks
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96 Wells
$230.00
In Stock
Application:
ELISA
Gene Symbol:
CSF2
Official Gene Full Name:
colony stimulating factor 2
NCBI Gene Id:
1437
Alias Symbols:
Colony-stimulating factor, CSF, GMCSF, GM-CSF, Granulocyte-macrophage colony-stimulating factor, MGC131935, MGC138897, Molgramostin, Sargramostim
Swissprot Id:
P04141
Protein Accession #:
NP_000749.2
Nucleotide Accession #:
NM_000758.3
Species Reactivity:
Human
Sample Type:
cell culture, supernates, serum, and plasma
Sensitivity:
7 pg/ml
Kit Reproducibility:
The coefficient of variation of both intra-assay and inter-assay were less than 10%.
Kit Component:
Components
2 vials human GM-CSF Standard lyophilized, 2000 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-human GM-CSF monoclonal antibody
2 vials Streptavidin-HRP solution,
1 bottle Standard /sample Diluent
1 bottle Biotin-Conjugate antibody Diluent
1 bottle Streptavidin-HRP Diluent
1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
1 vial Substrate Solution
1 vial Stop Solution
2 vials human IFN-gamma Standard lyophilized, 4000 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-human IFN-gamma antibody
Description of Target:
The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CSF2.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CSF2.
Predicted Homology Based on Immunogen Sequence:
Human
Datasheets / Downloads:
OKAA00005_96W
Specificity:
This assay recognizes both natural and recombinant human G-CSF. The factors listed below were prepared at 50ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.
Recombinant human: EGF, GM-CSF, FGF-4, FGF-5, FGF-6, FGF-9, FGF-10 ,
Recombinant mouse: FGF-8b, FGF-8c, G-CSF, VEGF-120, VEGF-164
Other proteins: bovine FGF acidic, bovine FGF basic, human PDGF, porcine PDGF
Assay Info:
Assay Method: Quantitative Sandwich ELISA
Additional Information:
Sample Type: cell culture supernates, serum, and plasma
:::
Reagents: REAGENTS
1. Aluminum pouches with a Microwell Plate coated with monoclonal antibody to human GM-CSF (8x12)
2. 2 vials human GM-CSF Standard lyophilized, 2000 pg/ml upon reconstitution
3. 2 vials concentrated Biotin-Conjugate anti-human GM-CSF monoclonal antibody
4. 2 vials Streptavidin-HRP solution,
5. 1 bottle Standard /sample Diluent
6. 1 bottle Biotin-Conjugate antibody Diluent
7. 1 bottle Streptavidin-HRP Diluent
8. 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
9. 1 vial Substrate Solution
10. 1 vial Stop Solution
11. 3 pieces Adhesive Films
12. package insert
Protein Name:
Granulocyte-macrophage colony-stimulating factor
Protocol:
Tips Information:
 
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