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Catalog No: OKEH03804
Size:96T
Price: $625.00
SKU
OKEH03804
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CSF2 ELISA Kit (Bovine) (OKEH03804) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CSF2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CSF2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CSF2 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Component
ComponentAmount
Anti-CSF2 Microplate96 Wells (12 x 8 Well strips)
CSF2 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated CSF2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.051 ng/mL
SpecificityNatural and recombinant Bovine Granulocyte-macrophage colony-stimulating factor
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCSF2
Gene Full Namecolony stimulating factor 2
Alias Symbolscolony stimulating factor 2 (granulocyte-macrophage);colony-stimulating factor;CSF;GMCSF;GM-CSF;granulocyte-macrophage colony-stimulating factor.
NCBI Gene Id281095
Protein NameP11052
Description of TargetGranulocyte-macrophage colony-stimulating factor
Uniprot IDhttps://www.uniprot.org/uniprot/P11052
Protein Accession #NP_776452.1
Nucleotide Accession #NM_174027.2
Protein Size (# AA)143
Molecular Weight16 kDa
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