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Catalog No: OKEH00160
Size:96T
Price: $425.00
SKU
OKEH00160
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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CSF1 ELISA Kit (Human) : 96 Wells (OKEH00160)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CSF1 ELISA Kit (Human) (OKEH00160) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CSF1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CSF1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CSF1 captured in well.
ELISA Kit Range31.2-2000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.5% (n = 20)
Mean Inter-assay CV%: < 8.0% (n = 20)
ELISA Kit Component
ComponentAmount
CSF1 Microplate96 Wells (12 x 8 Well strips)
CSF1 Lyophilized Standard2
100X Biotinylated CSF1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity12 pg/mL
SpecificityNatural and recombinant Human Macrophage colony-stimulating factor 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCSF1
Gene Full Namecolony stimulating factor 1
Alias Symbolscolony stimulating factor 1 (macrophage);CSF-1;lanimostim;macrophage colony stimulating factor 1;macrophage colony-stimulating factor 1;MCSF.
NCBI Gene Id1435
Protein NameP09603
Description of TargetMacrophage colony-stimulating factor 1
Uniprot IDhttps://www.uniprot.org/uniprot/P09603
Protein Accession #NP_000748
Nucleotide Accession #NM_000757
Protein Size (# AA)554
Molecular Weight60 kDa
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