Catalog No: OKCD01276 (Formerly GWB-KBBMG4)
Size:96WELLS
Price: $700.00
SKU
OKCD01276
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Datasheets/ManualsPrintable datasheet for CRAT ELISA Kit (Mouse) (OKCD01276)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carnitine Acetyltransferase (CRAT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carnitine Acetyltransferase (CRAT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carnitine Acetyltransferase (CRAT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Carnitine Acetyltransferase (CRAT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.78-50ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carnitine Acetyltransferase (CRAT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carnitine Acetyltransferase (CRAT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Crat Microplate96 Wells (12 x 8 Well Strips)
Crat Lyophilized Standard2 x 50 ng
100X Biotinylated Crat Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.31 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Carnitine Acetyltransferase (CRAT). No significant cross-reactivity or interference between Carnitine Acetyltransferase (CRAT) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCrat
Gene Full Namecarnitine acetyltransferase
Alias SymbolsAW107812;CA;CARAT;carnitine acetylase;Carnitine acetyltransferase;carnitine O-acetyltransferase;CAT.
NCBI Gene Id12908
Protein NameCarnitine O-acetyltransferase
Description of TargetCarnitine acetylase is specific for short chain fatty acids. Carnitine acetylase seems to affect the flux through the pyruvate dehydrogenase complex. It may be involved as well in the transport of acetyl-CoA into mitochondria.
Uniprot IDP47934
Protein Accession #NP_000746
Nucleotide Accession #NM_000755.3
Protein Size (# AA)626
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