Catalog No: OKCD00665 (Formerly GWB-KBBXK1)
Size:96WELLS
Price: $650.00
SKU
OKCD00665
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Datasheets/ManualsPrintable datasheet for CR2 ELISA Kit (Rat) (OKCD00665)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)98-105%97-105%78-97%95-103%
EDTA plasma(n=5)97-105%79-95%94-102%93-101%
heparin plasma(n=5)96-103%78-97%78-104%88-101%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Receptor 2 (CD21). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Receptor 2 (CD21). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Receptor 2 (CD21), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Complement Receptor 2 (CD21) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range1.56-100ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)87-10491
EDTA plasma(n=5)87-10497
heparin plasma(n=5)98-105102
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Receptor 2 (CD21) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Receptor 2 (CD21) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Cr2 Microplate96 Wells (12 x 8 Well Strips)
Cr2 Lyophilized Standard2 x 100 ng
100X Biotinylated Cr2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids.
Sensitivity< 0.57 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Complement Receptor 2 (CR2). No significant cross-reactivity or interference between Complement Receptor 2 (CR2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCr2
Gene Full Namecomplement C3d receptor 2
Alias SymbolsCd21;complement component (3d/Epstein Barr virus) receptor 2;complement component 3d receptor 2;complement receptor 2;complement receptor type 2.
NCBI Gene Id289395
Protein NameComplement C3d receptor 2
Description of TargetComplement C3d receptor 2
Uniprot IDD3Z9F7
Protein Accession #NP_001099459
Nucleotide Accession #NM_001105989.2
Protein Size (# AA)1029
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