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Catalog No: OKEH02699
Size:96T
Price: $475.00
SKU
OKEH02699
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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CR2 ELISA Kit (Human) : 96 Wells (OKEH02699)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CR2 ELISA Kit (Human) (OKEH02699) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CR2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for CR2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CR2 captured in well.
ELISA Kit Range0.78-50ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.1% (n = 20)
Mean Inter-assay CV%: < 8.2% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-CR2 Microplate96 Wells (12 x 8 Well strips)
CR2 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated CR2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.22 ng/mL
SpecificityNatural and recombinant Human Complement receptor type 2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCR2
Gene Full Namecomplement C3d receptor 2
Alias SymbolsC3DR;CD21;Complement C3d receptor;complement component (3d/Epstein Barr virus) receptor 2;complement component 3d receptor 2;complement receptor type 2;CR;CVID7;EBV receptor;Epstein-Barr virus receptor;SLEB9.
NCBI Gene Id1380
Protein NameP20023
Description of TargetComplement receptor type 2
Uniprot IDhttps://www.uniprot.org/uniprot/P20023
Protein Accession #NP_001006659.1
Nucleotide Accession #NM_001006658.2
Protein Size (# AA)1033
Molecular Weight113 kDa
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