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Catalog No: OKCD00699 (Formerly GWB-KBBXU4)
Size:96WELLS
Price: $675.00
SKU
OKCD00699
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Datasheets/ManualsPrintable datasheet for CPN2 ELISA Kit (Human) (OKCD00699)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)97-104%84-102%80-90%96-104%
EDTA plasma(n=5)88-96%87-94%86-97%80-96%
heparin plasma(n=5)81-88%90-98%78-93%95-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carboxypeptidase N2 (CPN2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carboxypeptidase N2 (CPN2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carboxypeptidase N2 (CPN2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Carboxypeptidase N2 (CPN2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range1.56-100ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)78-9490
EDTA plasma(n=5)78-9890
heparin plasma(n=5)95-10399
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carboxypeptidase N2 (CPN2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carboxypeptidase N2 (CPN2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CPN2 Microplate96 Wells (12 x 8 Well Strips)
CPN2 Lyophilized Standard2 x 100 ng
100X Biotinylated CPN2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids.
Sensitivity< 0.63 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Carboxypeptidase N2 (CPN2). No significant cross-reactivity or interference between Carboxypeptidase N2 (CPN2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCPN2
Gene Full Namecarboxypeptidase N subunit 2
Alias SymbolsACBP;carboxypeptidase N 83 kDa chain;carboxypeptidase N large subunit;Carboxypeptidase N polypeptide 2;carboxypeptidase N regulatory subunit;carboxypeptidase N subunit 2;carboxypeptidase N, polypeptide 2, 83kD.
NCBI Gene Id1370
Protein NameCarboxypeptidase N subunit 2
Description of TargetThe 83 kDa subunit binds and stabilizes the catalytic subunit at 37 degrees Celsius and keeps it in circulation. Under some circumstances it may be an allosteric modifier of the catalytic subunit.
Uniprot IDP22792
Protein Accession #NP_001073982
Nucleotide Accession #NM_001080513.3
Protein Size (# AA)545
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