CPB2 ELISA Kit (Human) : 96 Wells (OKCA00124)

Catalog No: OKCA00124

Size:96 Wells

Human CPB2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Linearity:

  Sample Type 1
  Sample Type	Dilution	Average %	Range %
  Human Serum (n=4)	1:200	98%	94-108%
  Human Serum (n=4)	1:400	96%	92-99%
  Human Serum (n=4)	1:800	95%	90-99%
  Human Serum (n=4)	1:1600	88%	84-96%

• ELISA Kit Principle: Aviva Systems Biology CPB2 ELISA Kit (Human) (OKCA00124) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for human CPB2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for human CPB2 is added, incubated and then followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP producing a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample human CPB2 captured in well.
• ELISA Kit Range: 31.25 ng/mL - 2,000 ng/mL
• ELISA Kit Recovery:

  Sample 1
  Sample Type	Average Recovery	Range
  Serum (n=5)	98%	92-104%

  Sample 2
  Sample Type	Average Recovery	Range
  EDTA Plasma (n=4)	92%	89-95%

• ELISA Kit Reproducibility: Intra-assay: CV% < 8% (n = 20).Inter-assay: CV% < 10% (n = 20)
• ELISA Kit Component:
  • Anti-CPB2 Microplate: 96 Well (12 x 8 Well Strips)
  • CPB2 Standard (Lyophilized): 2 x 2000 ng
  • 100X Biotinylated Anti-CPB2 Detector Antibody: 1 x 120 uL
  • 100X HRP-Avidin: 1 x 120 uL
  • Detection Antibody Diluent: 1 x 15 mL
  • HRP-Avidin Diluent: 1 x 15 mL
  • Sample Diluent: 1 x 50 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Plasma, Tissue Homogenates
• Sensitivity: < 7.81 ng/ml
• Specificity: This assay has high sensitivity and excellent specificity for detection of human
  TAFI. No significant cross-reactivity or interference between human TAFI and
  analogues was observed.
  Note: Limited by current skills and knowledge, it is impossible for us to complete
  the cross-reactivity detection between human TAFI and all the analogues,
  therefore, cross reaction may still exist.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: CPB2
• Gene Full Name: Carboxypeptidase B2
• Alias Symbols: CPU, PCPB, TAFI
• NCBI Gene Id: 1361
• Protein Name: Carboxypeptidase B2
• Description of Target: Carboxypeptidases are enzymes that hydrolyze C-terminal peptide bonds. The carboxypeptidase family includes metallo-, serine, and cysteine carboxypeptidases. According to their substrate specificity, these enzymes are referred to as carboxypeptidase A (cleaving aliphatic residues) or carboxypeptidase B (cleaving basic amino residues). The protein encoded by this gene is activated by trypsin and acts on carboxypeptidase B substrates. After thrombin activation, the mature protein downregulates fibrinolysis. Polymorphisms have been described for this gene and its promoter region. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]
• Uniprot ID: Q96IY4, Q15114, Q5T9K2
• Protein Accession #: NP_001265470
• Nucleotide Accession #: NM_001278541
• Protein Size (# AA): 423
• Molecular Weight: 48 kDa