CPA4 ELISA Kit (Human) (OKDD00203)

Catalog No: OKDD00203 (Formerly GWB-KBBTG4)

Size:96 Wells

Human CPA4 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked immunosorbent assay
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~4 Hours
• ELISA Kit Principle: Aviva Systems Biology CPA4 ELISA Kit (Human) (OKDD00203) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for CPA4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CPA4 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CPA4 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility:

  Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CPA4 were tested 20 times on one plate, respectively.

  Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CPA4 were tested on 3 different plates, 8 replicates in each plate.

  Intra-Assay: CV<10%

  Inter-Assay: CV<12%

• ELISA Kit Component:
  • Anti-CPA4 Microplate: 96 Wells (12 x 8 Well Strips)
  • CPA4 Lyophilized Standard: 2 x 10 ng
  • Standard Diluent: 1 x 20 mL
  • 100X CPA4 Biotinylated Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Detection Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Detection Conjugate Diluent: 1 x 12 mL
  • 10X Sample Diluent: 1 x 30 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma, tissue homogenates
• Sensitivity: < 0.051 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of CPA4. No significant cross-reactivity or interference between CPA4 and analogues was observed.
• Assay Info: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CPA4
• Gene Full Name: carboxypeptidase A4
• Alias Symbols: carboxypeptidase A3;carboxypeptidase A4;CPA3.
• NCBI Gene Id: 51200
• Protein Name: Carboxypeptidase A4
• Description of Target: Metalloprotease that could be involved in the histone hyperacetylation pathway (PubMed:10383164). Releases a C-terminal amino acid, with preference for -Phe, -Leu, -Ile, -Met, -Tyr and -Val (PubMed:20385563).
• Uniprot ID: Q9UI42
• Protein Accession #: NP_001156918.1
• Nucleotide Accession #: NM_001163446.1
• Protein Size (# AA): 421
• Molecular Weight: 47 kDa