CPA1 ELISA Kit (Rat) (OKCD00698)

Catalog No: OKCD00698 (Formerly GWB-KBBGN2)

Size:96WELLS

Rat Cpa1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	84-92%	97-105%	81-99%	98-105%
  EDTA plasma(n=5)	87-97%	95-103%	82-104%	96-104%
  heparin plasma(n=5)	85-99%	87-103%	89-103%	78-95%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carboxypeptidase A1, Pancreatic (CPA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carboxypeptidase A1, Pancreatic (CPA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carboxypeptidase A1, Pancreatic (CPA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Carboxypeptidase A1, Pancreatic (CPA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	81-90	86
  EDTA plasma(n=5)	88-95	91
  heparin plasma(n=5)	99-105	102

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carboxypeptidase A1, Pancreatic (CPA1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carboxypeptidase A1, Pancreatic (CPA1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Cpa1 Microplate: 96 Wells (12 x 8 Well Strips)
  • Cpa1 Lyophilized Standard: 2 x 100 ng
  • 100X Biotinylated Cpa1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids.
• Sensitivity: < 0.53 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Carboxypeptidase A1, Pancreatic (CPA1). No significant cross-reactivity or interference between Carboxypeptidase A1, Pancreatic (CPA1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Cpa1
• Gene Full Name: carboxypeptidase A1
• Alias Symbols: carboxypeptidase A1;carboxypeptidase A1 (pancreatic).
• NCBI Gene Id: 24269
• Protein Name: Carboxypeptidase A1
• Description of Target: Carboxypeptidase that catalyzes the release of a C-terminal amino acid, but has little or no action with -Asp, -Glu, -Arg, -Lys or -Pro.
• Uniprot ID: P00731
• Protein Accession #: NP_058694
• Nucleotide Accession #: NM_016998.3
• Protein Size (# AA): 419