AHR Western Blot Kit

Preview

Catalog#

Accession #

Size

Price

Qty

ARPK32243_T100

NP_001612

1 Kit

$549.00

Description:
The AHR Western Blot Kit provides all the reagents that are required for conducting high quality western blot analysis. Conditions and protocols have been optimized for easy visualization of the AHR protein target. Primary Target-Specific Antibody and Secondary HRP Conjugated Antibody are included to facilitate rapid detection of the target protein. The enclosed Positive Control Cell Lysate can be included in the western blot procedure in order to ensure that the antibody is functioning properly and successfully detecting the target protein. The peptide used to generate the primary antibody has also been enclosed to facilitate Peptide Blocking studies to confirm the specificity of the Primary Antibodies binding affinity.

# Western Blots / Kit:
25

Species Reactivity:
Human

Primary Antibody:
100µg AHR, Catalog# ARP32243_T100, Rabbit Polyclone, Protein A purifed, Predict MW 96kDa

Secondary Antibody:
4µg HRP Conjµgated Goat Anti-Rabbit IgG(AViHRP-GAR)

Positive Control Lysate:
625µg HepG2 Cell Lysate (AHL001)

Blocking Peptide:
100µg AHR Peptide(P03224)

50AA Region Containing 14AA Immunogen:
RLSVSYLRAKSFFDVALKSSPTERNGGQDNCRAANFREGLNLQEGEFLLQ

Reconstitution and Storage:
Primary Antibody 100µl dd H2O, Secondary Antibody 50µl dd H2O, Cell Lysate 125µl dd H2O, Peptide 100µl dd H2O. Working stock 4°, Long Term Storage -20° Aliquoted

Instructions:
Western Blot Kit Instruction Manual

Western Blot Protocol:
Western Blot Protocol

Peptide Blocking Protocol:
Western Blot Peptide Competition Assay Protocol

Peptide Datasheet:
P03224 Peptide Immunogen Data Sheet.pdf

Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Immunoblot

ARP32243-QC1712-WB.jpg