ABAT(4-aminobutyrate aminotransferase) Antibody

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Catalog#

Accession #

Size

Price

Qty

ARP51765_P050

NP_065737

50µg

$289.00

Description:
4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. 4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene.

Species Reactivity:
Human

Gene Symbol:
ABAT

Alias Symbols:
GABA-AT; GABAT; NPD009

Peptide ID:
P40140

ELISA titre using peptide based assay:
1:1562500

Search Keyword:
ABAT, GABA-AT; GABAT; NPD009, NP_065737

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Antigen Specificity:
Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human ABAT with an internal ID of P40140

Reconstitution and Storage:
Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 μl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.

Key Reference:
Inada,T., (2008) Pharmacogenet. Genomics 18 (4), 317-323

Quality Control:
Rabbit Ig G is purified by peptide affinity chromatography method.

Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Western Blot
Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody.

Immunoblot

ARP51765-QC20987-WB.jpg

Protein Accession#	Swissprot Id	Nucleotide Accession#	Protein Size	Molecular Weight
NP_065737	P80404	NM_020686	500	55kDa