Home > Products >  Antibodies
Home > Products >Antibodies :   Cell Cycle Proteins
Home > Products >Antibodies :   Protein Kinases

AURKA(aurora kinase A) Antibody

Preview
Catalog#   Accession # Size Price Qty
ARP30876_P050   NP_003591 50µg $289.00
Description: 
AURKA is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. It is found at the centrosome in interphase cells and at the spindle poles in mitosis. This protein may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene.The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene.
Gene Symbol: 
AURKA  
Alias Symbols: 
AIK; ARK1; AURA; AURORA2; BTAK; MGC34538; STK15; STK6; STK7  
Peptide ID: 
P01547  
ELISA titre using peptide based assay: 
1:62500  
Search Keyword: 
AURKA, AIK; ARK1; AURA; AURORA2; BTAK; MGC34538; STK15; STK6; STK7, NP_003591  
Click Here to view more information (protein size, molecular weight, etc)
Species Reactivity: 
(Primary - WB; Secondary - Computational Homology)
Human 
Antigen Specificity: 
Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human AURKA with an internal ID of P01547 
Reconstitution and Storage: 
Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 μl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles. 
Key Reference: 
Ye,Y., (2008) Cancer 112 (11), 2467-2474 
Quality Control: 
Rabbit Ig G is purified by peptide affinity chromatography method. 
Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.
Western Blot
Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody.
Immunoblot

ARP30876-QC0573-WB.jpg
 
Protein Accession# Swissprot Id Nucleotide Accession# Protein Size Molecular Weight
NP_003591 O14965 NM_003600 403 46kDa