Catalog No: OKCD00680 (Formerly GWB-KBBQO2)
Size:96WELLS
Price: $725.00
SKU
OKCD00680
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Datasheets/ManualsPrintable datasheet for COL9A1 ELISA Kit (Rat) (OKCD00680)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type IX Alpha 1 (COL9a1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type IX Alpha 1 (COL9a1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type IX Alpha 1 (COL9a1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Collagen Type IX Alpha 1 (COL9a1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range1.56-100ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type IX Alpha 1 (COL9a1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type IX Alpha 1 (COL9a1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Col9a1 Microplate96 Wells (12 x 8 Well Strips)
Col9a1 Lyophilized Standard2 x 100 ng
100X Biotinylated Col9a1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.58 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Collagen Type IX Alpha 1 (COL9a1). No significant cross-reactivity or interference between Collagen Type IX Alpha 1 (COL9a1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCol9a1
Gene Full Namecollagen type IX alpha 1 chain
Alias Symbolscollagen alpha-1(IX) chain;collagen, type IX, alpha 1;procollagen, type IX, alpha 1.
NCBI Gene Id305104
Protein NameCollagen alpha-1(IX) chain
Description of TargetCollagen alpha-1(IX) chain
Uniprot IDP20850
Protein Accession #NP_001094312
Nucleotide Accession #NM_001100842.1
Protein Size (# AA)325
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