COL5A2 ELISA Kit (Human) (OKCD00862)

Catalog No: OKCD00862 (Formerly GWB-KBBUT4)

Size:96WELLS

Human COL5A2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	91-105%	80-93%	94-103%	97-105%
  EDTA plasma(n=5)	95-104%	96-104%	90-98%	78-90%
  heparin plasma(n=5)	87-94%	82-102%	90-99%	87-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type V Alpha 2 (COL5a2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type V Alpha 2 (COL5a2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type V Alpha 2 (COL5a2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Collagen Type V Alpha 2 (COL5a2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	86-103	92
  EDTA plasma(n=5)	80-94	88
  heparin plasma(n=5)	83-94	86

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type V Alpha 2 (COL5a2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type V Alpha 2 (COL5a2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-COL5A2 Microplate: 96 Wells (12 x 8 Well Strips)
  • COL5A2 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated COL5A2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 0.126 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Collagen Type V Alpha 2 (COL5a2). No significant cross-reactivity or interference between Collagen Type V Alpha 2 (COL5a2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: COL5A2
• Gene Full Name: collagen type V alpha 2 chain
• Alias Symbols: AB collagen;collagen alpha-2(V) chain;collagen, fetal membrane, A polypeptide;EDSC;EDSCL2;type V preprocollagen alpha 2 chain.
• NCBI Gene Id: 1290
• Protein Name: Collagen alpha-2(V) chain
• Description of Target: Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. Type V collagen is a key determinant in the assembly of tissue-specific matrices (By similarity).
• Uniprot ID: P05997
• Protein Accession #: NP_000384
• Nucleotide Accession #: NM_000393.3
• Protein Size (# AA): 1499