COL4A1 ELISA Kit (Mouse) (OKCD00222)

Catalog No: OKCD00222 (Formerly GWB-KBBFH9)

Size:96WELLS

Mouse Col4a1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	92-99%	90-104%	90-105%	80-89%
  EDTA plasma(n=5)	79-94%	93-102%	97-105%	92-101%
  heparin plasma(n=5)	84-99%	97-104%	82-93%	96-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type IV Alpha 1 (COL4a1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type IV Alpha 1 (COL4a1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type IV Alpha 1 (COL4a1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Collagen Type IV Alpha 1 (COL4a1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	91-101	95
  EDTA plasma(n=5)	78-103	80
  heparin plasma(n=5)	79-104	81

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type IV Alpha 1 (COL4a1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type IV Alpha 1 (COL4a1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Col4a1 Microplate: 96 Wells (12 x 8 Well Strips)
  • Col4a1 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated Col4a1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.058 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Collagen Type IV Alpha 1 (COL4a1). No significant cross-reactivity or interference between Collagen Type IV Alpha 1 (COL4a1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Col4a1
• Gene Full Name: collagen, type IV, alpha 1
• Alias Symbols: alpha-1 type IV collagen;alpha1(IV) collagen;Br;Bru;Col4;Col4a-1;collagen alpha-1(IV) chain;Del(8)44;procollagen, type IV, alpha 1;R;Raw;S;Svc.
• NCBI Gene Id: 12826
• Protein Name: Collagen alpha-1(IV) chain
• Description of Target: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
• Uniprot ID: P02463
• Protein Accession #: NP_034061
• Nucleotide Accession #: NM_009931.2
• Protein Size (# AA): 1669