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Catalog No: OKEH00269
Size:96T
Price: $525.00
SKU
OKEH00269
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COL3A1 ELISA Kit (Human) : 96 Wells (OKEH00269)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH00269
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology COL3A1 ELISA Kit (Human) (OKEH00269) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for COL3A1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for COL3A1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample COL3A1 captured in well.
ELISA Kit Range3.12-200ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 92%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 3.9% (n = 20)
Mean Inter-assay CV%: < 7.1% (n = 20)
ELISA Kit Component
ComponentAmount
COL3A1Microplate96 Wells (12 x 8 Well strips)
COL3A1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated COL3A1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity1.2 ng/mL
SpecificityNatural and recombinant Human Collagen alpha-1(III) chain
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCOL3A1
Gene Full Namecollagen type III alpha 1 chain
Alias Symbolsalpha1 (III) collagen;alpha-1 type III collagen;collagen alpha-1(III) chain;collagen, fetal;collagen, type III, alpha 1;EDS4A;EDSVASC;Ehlers-Danlos syndrome type IV, autosomal dominant;PMGEDSV.
NCBI Gene Id1281
Protein NameP02461
Description of TargetCollagen alpha-1(III) chain
Uniprot IDhttps://www.uniprot.org/uniprot/P02461
Protein Accession #NP_000081.1
Nucleotide Accession #NM_000090.3
Protein Size (# AA)1466
Molecular Weight139 kDa
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