COL10A1 ELISA Kit (Mouse) (OKCD02455)

Catalog No: OKCD02455

Size:96WELLS

Mouse COL10A1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	80-96%	88-102%	90-102%	80-94%
  EDTA Plasma (n=5)	95-102%	92-101%	85-101%	83-91%
  Heparin Plasma (n=5)	94-104%	83-102%	99-105%	78-89%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type X (COL10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type X (COL10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type X (COL10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Collagen Type X (COL10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 3.12-200ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	79-102	84
  EDTA Plasma (n=5)	93-101	97
  Heparin Plasma(n=5)	90-99	93

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type X (COL10) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type X (COL10) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-COL10A1 Microplate: 96 Wells (12 x 8 Well strips)
  • COL10A1 Lyophilized Standard: 2
  • 100X Biotinylated COL10A1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 0.115 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Collagen Type X (COL10). No significant cross-reactivity or interference between Collagen Type X (COL10) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Col10a1
• Gene Full Name: collagen, type X, alpha 1
• Alias Symbols: Col;Col10;Col10a-1;collagen alpha-1(X) chain;procollagen, type X, alpha 1.
• NCBI Gene Id: 12813
• Protein Name: Collagen alpha-1(X) chain
• Description of Target: Type X collagen is a product of hypertrophic chondrocytes and has been localized to presumptive mineralization zones of hyaline cartilage.
• Uniprot ID: Q05306
• Protein Accession #: NP_034055.1
• Nucleotide Accession #: NM_009925.4
• Protein Size (# AA): 680