Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKCD02452
Size:96WELLS
Price: $700.00
SKU
OKCD02452
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for CLC ELISA Kit (Mouse) (OKCD02452)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)81-91%87-98%92-101%88-95%
EDTA Plasma (n=5)94-101%78-102%82-94%97-105%
Heparin Plasma (n=5)89-97%94-101%78-103%89-102%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Charcot Leyden Crystal Protein (CLC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Charcot Leyden Crystal Protein (CLC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Charcot Leyden Crystal Protein (CLC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Charcot Leyden Crystal Protein (CLC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10291
EDTA Plasma (n=5)98-105101
Heparin Plasma(n=5)89-9693
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Charcot Leyden Crystal Protein (CLC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Charcot Leyden Crystal Protein (CLC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CLC Microplate96 Wells (12 x 8 Well strips)
CLC Lyophilized Standard2
100X Biotinylated CLC Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernatant and other biological fluids
Sensitivity0.054 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Charcot Leyden Crystal Protein (CLC). No significant cross-reactivity or interference between Charcot Leyden Crystal Protein (CLC) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolCLC
Gene Full NameCharcot-Leyden crystal protein
Alias SymbolsCharcot-Leyden crystal protein homolog.
Protein NameGalectin-10
Description of TargetRegulates immune responses through the recognition of cell-surface glycans. Essential for the anergy and suppressive function of CD25-positive regulatory T-cells (Treg) (By similarity).
Uniprot IDP97400
Protein Size (# AA)68
Write Your Own Review
You're reviewing:CLC ELISA Kit (Mouse) (OKCD02452)
Your Rating
We found other products you might like!