CHRNA4 Antibody (OAEB02107)

Catalog No: OAEB02107 (Formerly GWB-EV0364)

Size:100UG

OAEB02107 (0.3ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Info

• Predicted Species Reactivity: Bovine|Dog|Human|Mouse|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB
  Description: This antibody is expected NOT to cross-react with the similar alpha 2 subunit.
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: CHRNA4
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 93%; Guinea Pig: 92%; Human: 100%; Mouse: 92%; Rabbit: 91%; Rat: 92%
• Peptide Sequence: C-HVETRAHAEERLLKK
• Concentration: 0.5 mg/ml
• Application Info: Peptide ELISA: antibody detection limit dilution 1:64000.
  Western Blot: Approx 70kDa band observed in Rat Brain lysates (calculated MW of 70.0kDa according to Human NP_000735.1and of 70.2kDa according to Rat NP_077330.1). Recommended concentration: 1-3ug/ml.
  An additional band of unknown identity was also consistently observed at 22kDa. This band was successfully blocked by incubation with the immunising peptide.
• Reference: * Schwarz J, Schwarz SC, Dorigo O, Stutzer A, Wegner F, Labarca C, Deshpande P, Gil JS, Berk AJ, Lester HA.; Enhanced expression of hypersensitive alpha4* nAChR in adult mice increases the loss of midbrain dopaminergic neurons.; FASEB J. 2006 May;20(7):935-46.; Pubmed 16675851

Target Info

• Gene Symbol: Chrna4|CHRNA4
• Gene Full Name: cholinergic receptor nicotinic alpha 4 subunit|cholinergic receptor, nicotinic, alpha polypeptide 4
• Alias Symbols: a4 nicotinic receptor;Ac;acetylcholine receptor alpha 4 neural;acetylcholine receptor, neuronal, nicotinic, alpha-4 subunit;Acr;Acra4;Acra-4;alpha4 nAChR;BFNC;cholinergic receptor, nicotinic alpha 4;cholinergic receptor, nicotinic, alpha 4 (neuronal);cholinergic receptor, nicotinic, alpha polypeptide 4;EBN;EBN1;ENFL1;NACHR;NACHRA4;NACRA4;NARAC;neuronal acetylcholine receptor subunit alpha-4;Neuronal nicotinic acetylcholine receptor alpha 4 subunit;neuronal nicotinic acetylcholine receptor alpha-4 subunit.
• NCBI Gene Id: 1137|11438|25590
• Protein Name: Neuronal acetylcholine receptor subunit alpha-4
• Uniprot ID: P43681
• Protein Accession #: NP_000735.1
• Molecular Weight: 70