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Catalog No: OKEH02028
Size:96T
Price: $600.00
SKU
OKEH02028
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CHID1 ELISA Kit (Human) (OKEH02028) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human CHID1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated CHID1 and incubated. The CHID1 found in the sample or standards competes with the biotinylated CHID1 for limited binding sites on the immobilized anti-human CHID1 antibody. Excess unbound biotinylated CHID1 and sample or standard CHID1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated CHID1 captured in the well and inversely proportional to the amount of CHID1 which was contained in the sample or standard.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.5% (n = 20)
Mean Inter-assay CV%: < 8.9% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-CHID1 Microplate96 Wells (12 x 8 Well strips)
CHID1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X CHID1-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.12 ng/mL
SpecificityNatural and recombinant Human Chitinase domain-containing protein 1
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolCHID1
Gene Full Namechitinase domain containing 1
Alias Symbolschitinase domain-containing protein 1;GL008;SICLP;SI-CLP;stabilin-1 interacting chitinase-like protein;Stabilin-1-interacting chitinase-like protein.
NCBI Gene Id66005
Protein NameQ9BWS9
Description of TargetChitinase domain-containing protein 1
Uniprot IDhttps://www.uniprot.org/uniprot/Q9BWS9
Protein Accession #NP_001136146.1
Nucleotide Accession #NM_001142674.1
Protein Size (# AA)393
Molecular Weight44 kDa
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