CHI3L2 ELISA Kit (Human) (OKCD01055)

Catalog No: OKCD01055 (Formerly GWB-KBBFI9)

Size:96WELLS

Human CHI3L2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	81-89%	89-104%	78-90%	80-102%
  EDTA plasma(n=5)	80-101%	97-104%	88-99%	84-103%
  heparin plasma(n=5)	78-96%	81-92%	78-90%	96-103%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chitinase 3 Like Protein 2 (CHI3L2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chitinase 3 Like Protein 2 (CHI3L2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chitinase 3 Like Protein 2 (CHI3L2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Chitinase 3 Like Protein 2 (CHI3L2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	88-95	92
  EDTA plasma(n=5)	83-96	89
  heparin plasma(n=5)	92-99	95

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chitinase 3 Like Protein 2 (CHI3L2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chitinase 3 Like Protein 2 (CHI3L2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CHI3L2 Microplate: 96 Wells (12 x 8 Well Strips)
  • CHI3L2 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated CHI3L2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 0.133 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Chitinase 3 Like Protein 2 (CHI3L2). No significant cross-reactivity or interference between Chitinase 3 Like Protein 2 (CHI3L2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: CHI3L2
• Gene Full Name: chitinase 3 like 2
• Alias Symbols: CHIL2;chitinase-3-like protein 2;chondrocyte protein 39;YKL39;YKL-39.
• NCBI Gene Id: 1117
• Protein Name: Chitinase-3-like protein 2
• Description of Target: Lectin that binds chitooligosaccharides and other glycans with high affinity, but not heparin. Has no chitinase activity.
• Uniprot ID: Q15782
• Protein Accession #: NP_001020368
• Nucleotide Accession #: NM_001025197.1
• Protein Size (# AA): 390